Abstract:

To ascertain the effect of different culture conditions on diversity of endophytic fungi from Oxytropis kansuensis in China, the study collected Oxytropis kansuensis samples in Qilian county of Qinghai province, using the method of surface disinfection and different medium, to isolate and purify the endophytic fungi in stems and leafs, then the morphology techniques and ITS sequence analysis were used to identify their species, and calculated diversity index by Biodap. The results showed that 433 fungal colonies were isolated from 629 samples, which were combined to 28 kinds of different endophytic fungus. Undifilum oxytropis was the dominant strain, and advantage was more obvious in the light condition. The Shannon-Weiner index was 1.99, evenness index was 0.60, simpson’ diversity index (D) was 0.257. The diversity was different under different culture conditions. The Shannon-Weiner index was highest in the dark condition, and the simpson’ diversity index was biggest in the light condition. The results showed that the diversity and isolation rate of endophytic fungi from Qilian county of Qinghai province was different under different culture conditions. Dark condition and PDA medium could improve the isolation rate of endophytic fungi, dark condition and BDA medium can increase the diversity, as the Undifilum oxytropis, the light condition and BDA medium can increase the separation rate.